Journal
BIOMATERIALS
Volume 27, Issue 15, Pages 2944-2950Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2006.01.013
Keywords
PHB; PHBHHx; smooth muscle cells; alpha-actin; crystallization; cell cycle
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In this paper, comprehensive characteristics including cell attachment, cell proliferation status, cell cycle progression and phenotypic changes of smooth muscle cells from rabbit aorta (RaSMCs) were studied on poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) containing 0-20% HHx (mol%) in comparison with tissue culture plates (TCPs). Results demonstrated that RaSMCs adhered better oil PHBHHx containing 12% HHx (12% HHx) although they proliferated better on 20% HHx-containing PHBHHx films (20% HHx). This was explained by the difference in cell cycle progression observed using flow cytometry, as it was found that only 20% HHx-containing polymer could maintain the normal cell cycle evolution as TCPs did after 3d incubation. The highest expression level and typical spindle-like distribution of alpha-actin on 20% HHx-containin polymer were characterized as the contractile-like phenotype, suggesting that RaSMCs tended to differentiate rather than proliferate compared to the cells grown on 12% HHx polymer. Results obtained above suggested that 20% HHx was suitable for RaSMCs proliferation, leading to its change to contractile phenotype. This study extends the potential applications of PHBHHx in SMCs-related graft scaffold fabrication for tissue engineering. (c) 2006 Elsevier Ltd. All rights reserved.
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