Journal
FEMS MICROBIOLOGY LETTERS
Volume 258, Issue 1, Pages 55-60Publisher
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2006.00196.x
Keywords
arcA; toluidine blue; PHB; O-2 consumption; ROS
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arcA codes for a central regulator in Escherichia coli that responds to redox conditions of growth. Mutations in this gene, originally named dye, confer sensitivity to toluidine blue and other redox dyes. However, the molecular basis for the dye-sensitive phenotype has not been elucidated. In this work, we show that toluidine blue redirects electrons to O-2 and causes an increase in the generation of reactive O-2 species (ROS). We also demonstrate that synthesis of poly (3-hydroxybutyrate) suppresses the Dye phenotype in E. coli recombinants, as the capacity to synthesize the polymer reduces sensitivity to toluidine blue, O-2 consumption and ROS production levels.
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