Journal
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
Volume 290, Issue 5, Pages H1818-H1825Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpheart.01062.2005
Keywords
norepinephrine; neurotransmitter release; cotransmission; adenosine 5 '-triphosphate
Funding
- NHLBI NIH HHS [HL-60031, R01 HL060031] Funding Source: Medline
Ask authors/readers for more resources
Using high-performance liquid chromatography techniques with fluorescence and electrochemical detection, we found that beta-nicotinamide adenine dinucleotide (beta-NAD) is released in response to electrical field stimulation ( 4 - 16 Hz, 0.3 ms, 15 V, 120 s) along with ATP and norepinephrine ( NE) in the canine isolated mesenteric arteries. The release of beta-NAD increases with number of pulses/stimulation frequencies. Immunohistochemistry analysis showed dense distribution of tyrosine hydroxylase-like immunoreactivity (TH-LI) and sparse distribution of TH-LI-negative nerve processes, suggesting that these blood vessels are primarily under sympathetic nervous system control with some contribution of other ( e. g., sensory) neurons. Exogenous NE ( 3 mu mol/l), alpha,beta-methylene ATP ( 1 mu mol/l), neuropeptide Y (NPY, 0.1 mu mol/l), CGRP (0.1 mu mol/l), vasoactive intestinal peptide ( VIP, 0.1 mu mol/l), and substance P (SP, 0.1 mu mol/l) had no effect on the basal release of beta-NAD, suggesting that the overflow of beta-NAD is evoked by neither the sympathetic neurotransmitters NE, ATP, and NPY, nor the neuropeptides CGRP, VIP, and SP. Botulinum neurotoxin A (BoNTA, 0.1 mu mol/l) abolished the evoked release of NE, ATP, and beta-NAD at 4 Hz, suggesting that at low levels of neural activity, release of these neurotransmitters results from N-ethylmaleimide- sensitive factor attachment protein receptor/synaptosomal- associated protein of 25 kDa-mediated exocytosis. At 16 Hz, however, the evoked release of NE, ATP, and beta-NAD was reduced by BoNTA by similar to 90, 60, and 80%, respectively, suggesting that at higher levels of neural activity, beta-NAD is likely to be released from different populations of synaptic vesicles or different populations of nerve terminals (i.e., sympathetic and sensory terminals).
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available