Journal
STRUCTURE
Volume 14, Issue 5, Pages 869-880Publisher
CELL PRESS
DOI: 10.1016/j.str.2006.03.009
Keywords
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Funding
- NCI NIH HHS [T32 CA80416] Funding Source: Medline
- NIGMS NIH HHS [GM49857] Funding Source: Medline
- PHS HHS [T32 G08268] Funding Source: Medline
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Homing endonucleases are highly specific catalysts of DNA strand breaks, leading to the transfer of mobile intervening sequences containing the endonuclease ORF. We have determined the structure and DNA recognition behavior of I-Ceul, a homodimeric LAGLI-DADG endonuclease from Chlamydomonas eugametos. This symmetric endonuclease displays unique structural elaborations on its core enzyme fold, and it preferentially cleaves a highly asymmetric target site. This latter property represents an early step, prior to gene fusion, in the generation of asymmetric DNA binding platforms from homodimeric ancestors. The divergence of the sequence, structure, and target recognition behavior of homing endonucleases, as illustrated by this study, leads to the invasion of novel genomic sites by mobile introns during evolution.
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