Journal
CARBOHYDRATE RESEARCH
Volume 341, Issue 6, Pages 766-775Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2005.12.013
Keywords
Rhodococcus rhodochrous; extracellular polysaccharide; bioremediation; emulsification; structure; NMR spectroscopy
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A possibility has been suggested of applying the EPS produced by Rhodococcus rhodochrous strain S-2 (S-2 EPS) to the bioremediation of oil-contaminated environments, because its addition, together with minerals, to oil-contaminated seawater resulted in emulsification of the oil, increased the degradation of polyaromatic hydrocarbons (PAH) of the oil, and led to the dominance of PAH-degrading marine bacteria. To understand the underlying principles of these phenomena, we determined the chemical structure of the sugar chain of S-2 EPS. The EPS was found to be composed Of D-galactose, D-mannose, D-glucose, and D-glucuronic acid, in a molar ratio of 1:1:1:1. In addition, 0.8% (w/w) of octadecanoic acid and 2.7% (w/w) of hexadecanoic acid were also contained in its structure. By H-1 and C-13 NMR spectroscopy, including 2D DQF-COSY, TOCSY, HMQC, HMBC, and NOESY experiments, as well as chemical and enzymatic analyses, the polysaccharide was shown to consist of tetrasaccharide repeating units with the following structure: beta-D-GlcpA 1 down arrow 2 -> 3)-alpha-D-Manp-(1 -> 3)-alpha-D-Glcp-(1 -> 3)-alpha-D-Galp-(1 -> (c) 2006 Elsevier Ltd. All rights reserved.
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