Journal
ANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY
Volume 117, Issue 9, Pages 696-702Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/000348940811700912
Keywords
extracellular matrix; hepatocyte growth factor; hyaluronan synthase; procollagen; transforming growth factor-beta 1; vocal fold wound healing
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Objectives: We performed a prospective, sham-controlled animal study to investigate the effects of hepatocyte growth factor (HGF) manipulation of the extracellular matrix on vocal fold gene expression during acute injury. Methods: Bilateral vocal fold wounds were created in 40 rats. The rats were randomly assigned to I of 2 groups (sham treatment or HGF treatment) and received treatment of the injured area at the time of wounding and on alternate post-treatment days. The injured vocal fold specimens were harvested on post-treatment days 1, 3, 7, and 14. We used real-time reverse transcription polymerase chain reaction to quantify messenger RNA expression of transforming growth factor (TGF)-beta 1, procollagen types I and III, hyaluronan synthase (HAS)-1, HAS-2, and HAS-3. Results: A multivariate analysis of variance revealed a significant interaction between treatment group and post-treatment day for TGF-beta 1, procollagen type I, procollagen type III, and HAS-2. Post hoc testing revealed significantly lower expression of procollagen type III and significantly higher expression of HAS-2 on post-treatment day 14 in the HGF treatment group than in the sham treatment group. Conclusions: Results provide evidence of HGF treatment effects on procollagen type III and HAS-2 gene expression pathways.
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