Journal
FRONTIERS IN BIOSCIENCE-LANDMARK
Volume 11, Issue -, Pages 1690-1695Publisher
FRONTIERS IN BIOSCIENCE INC
DOI: 10.2741/1914
Keywords
human; chondrocytes; tissue-engineering; articular cartilage-constructs
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Tissue-engineering is considered a promising avenue for developing human articular cartilage implants that can be employed for resurfacing damaged cartilage in the early stages of osteoarthritis. In the present study, human cartilage-constructs were produced from human osteoarthritic chondrocytes maintained on a scaffold of HYAFF (R)-11 in perfusion mini-bioreactors or after implantation and recovery from nude or SCID mice after 3 weeks. The human cartilage-construct extracellular matrix reacted positively with anti-Type II collagen monoclonal antibody, but not with anti-Type I or anti-Type X collagen monoclonal antibodies. A significant portion of the cartilage-construct extracellular matrix stained metachromatic with Toluidine blue-O indicative of sulfated-proteoglycan deposition. Cyclic hydrostatic pressure applied for 4 hrs at 5 MPa using a 1 Hertz sinusoidal frequency significantly increased ( p < 0.02) the proportion of apoptotic cells in the cartilage-constructs ( 41% +/- 4.2%; mean +/- SD) compared to control cartilage-constructs ( 28.5 +/- 8.4%).
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