Journal
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
Volume 85, Issue 1, Pages 1-6Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pestbp.2005.09.001
Keywords
wheat cytochrome P450; heterologous expression; herbicide metabolism; sulfonylurea herbicides
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A wheat cytochrome P450 cDNA (CYP71C6v1) was cloned by RT-PCR and heterologously expressed in yeast. The microsomal fractions derived from this strain could catalyze the metabolism of some sulfonylurea herbicides such as chlorsulfuron, triasulfuron, metsulfuron-metyl, bensulfuron-metyl, and tribenuron-rnetyl, but not sulfonylurea herbicides such as thifensulfuron and pyrazosulfuron. Kinetic parameters K-m for chlorsulfuron and triasulfuron were 57 (+/- 15)mu M and 38 (+/- 16) mu M in vitro, respectively. Analysis of the metabolites demonstrated that the CYP71C6v1 functioned as a 5-phenyl ring hydroxylase when chlorsulfuron and triasulfuron were the substrates. (c) 2005 Elsevier Inc. All rights reserved.
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