Journal
FASEB JOURNAL
Volume 20, Issue 7, Pages 916-925Publisher
FEDERATION AMER SOC EXP BIOL
DOI: 10.1096/fj.05-5129com
Keywords
Rho kinase; pressure overload; fibrosis; hypertrophy; fibrogenic cytokines
Categories
Funding
- NHLBI NIH HHS [R01-HL64356, R01 HL072897-04, R01 HL072897, P01 HL085098-01A10002, P01-HL49953, P01-HL42550, P01 HL085098, R01-HL72897] Funding Source: Medline
- NIA NIH HHS [R01-AG18599] Funding Source: Medline
Ask authors/readers for more resources
Ventricular myocyte hypertrophy is an important compensatory growth response to pressure overload. However, pathophysiological cardiac hypertrophy is accompanied by reactive fibrosis and remodeling. The Rho kinase family, consisting of ROCK1 and ROCK2, has been implicated in cardiac hypertrophy and ventricular remodeling. However, these previous studies relied heavily on pharmacological inhibitors, and not on gene deletion. Here we used ROCK1 knockout (ROCK1(-/-)) mice to investigate role of ROCK1 in the development of ventricular remodeling induced by transverse aortic banding. We observed that ROCK1 deletion did not impair compensatory hypertrophic response induced by pressure overload. However, ROCK1(-/-) mice exhibited reduced perivascular and interstitial fibrosis, which was observed at 3 wk but not at 1 wk after the banding. The reduced fibrosis in the myocardium of ROCK1(-/-) mice was closely associated with reduced expression of a variety of extracellular matrix (ECM) proteins and fibrogenic cytokines such as TGF beta 2 and connective tissue growth factor. This inhibitory effect of ROCK1 deletion on pathophysiological induction of fibrogenic cytokines was further confirmed in the myocardium of transgenic mice with cardiomyocyte-specific overexpression of G alpha q. Thus, these results indicate that ROCK1 contributes to the development of cardiac fibrosis and induction of fibrogenic cytokines in cardiomyocytes in response to pathological stimuli.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available