Journal
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
Volume 39, Issue 1-4, Pages 156-159Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molcatb.2006.01.036
Keywords
high-throughput screenings nitrilase; UV-vis spectroscopy; enantioselectivity
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In this paper we describe a colorimetric method for the screening of nitrilases. When a buffered solution of CoCl2 is added to a nitrilase-catalyzed hydrolysis reaction, the ammonia product forms a complex with the cobalt ion resulting in a color change from light pink to yellow, which can readily be quantified using a spectrophotometer at 375 nm. This method has been demonstrated for both wild-type and evolved nitrilases. (c) 2006 Elsevier B.V. All rights reserved.
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