Journal
ANNALS OF ONCOLOGY
Volume 25, Issue 10, Pages 1959-1965Publisher
OXFORD UNIV PRESS
DOI: 10.1093/annonc/mdu288
Keywords
breast cancer; circulating tumor DNA; targeted gene sequencing; liquid biopsy
Categories
Funding
- Fonds de la recherche scientifique (FNRS), from Breast Cancer Research Foundation (BCRF)
- MEDIC Foundation
- OncoDNA
- 'Les Amis de l'Institut Bordet'
- FNRS
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Background: Molecular screening programs use next-generation sequencing (NGS) of cancer gene panels to analyze metastatic biopsies. We interrogated whether plasma could be used as an alternative to metastatic biopsies. Patients and methods: The Ion AmpliSeq (TM) Cancer Hotspot Panel v2 (Ion Torrent), covering 2800 COSMIC mutations from 50 cancer genes was used to analyze 69 tumor (primary/metastases) and 31 plasma samples from 17 metastatic breast cancer patients. The targeted coverage for tumor DNA was x1000 and for plasma cell-free DNA x25 000. Whole blood normal DNAwas used to exclude germline variants. The Illumina technology was used to confirm observed mutations. Results: Evaluable NGS results were obtained for 60 tumor and 31 plasma samples from 17 patients. When tumor samples were analyzed, 12 of 17 (71%, 95% confidence interval (CI) 44% to 90%) patients had >= 1 mutation (median 1 mutation per patient, range 0-2 mutations) in either p53, PIK3CA, PTEN, AKT1 or IDH2 gene. When plasma samples were analyzed, 12 of 17 (71%, 95% CI: 44-90%) patients had = 1 mutation (median 1 mutation per patient, range 0-2 mutations) in either p53, PIK3CA, PTEN, AKT1, IDH2 and SMAD4. All mutations were confirmed. When we focused on tumor and plasma samples collected at the same time-point, we observed that, in four patients, no mutation was identified in either tumor or plasma; in nine patients, the same mutations was identified in tumor and plasma; in two patients, a mutation was identified in tumor but not in plasma; in two patients, a mutation was identified in plasma but not in tumor. Thus, in 13 of 17 (76%, 95% CI 50% to 93%) patients, tumor and plasma provided concordant results whereas in 4 of 17 (24%, 95% CI 7% to 50%) patients, the results were discordant, providing complementary information. Conclusion: Plasma can be prospectively tested as an alternative to metastatic biopsies in molecular screening programs.
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