Synthesis and antiproliferative activity of two new tiazofurin analogues with 2′-amido functionalities

Two novel tiazofurin analogues 2 and 3 were synthesized starting from D-glucose. The key step of the synthesis was the efficient one-step hydrogen sulfide-mediated conversion of 2-azido-3-O-acyl-ribofuranosyl cyanides to the corresponding 2-amido thiocarboxamides. Compounds 2 and 3 were evaluated for their in vitro antiproliferative activity against certain human tumour cell lines. Remarkably, compound 2 was found to be 570-fold more potent than tiazofurin against MCF-7 cells, while compound 3 showed the most powerful cytotoxicity against HT-29 cancer cells, being almost 100-fold more active than tiazofurin. (C) 2006 Elsevier Ltd. All rights reserved.