Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 836, Issue 1-2, Pages 92-97Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2006.03.044
Keywords
polycyclic aromatic hydrocarbons; metabolites; 1-hydroxypyrene; 3-hydroxybenzo[a]pyrene; liquid chromatography-tandem mass spectrometry; urine
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A fast analysis method using liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometry was developed for the simultaneous determination of the 1-hydroxypyrene (1-OHP) and 3-hydroxybenzo[a]pyrene (3-OHBaP) in urine. Mass transitions were monitored at m/z 219.3-200.0 for 1-OHP and m/z 269.2-252.2 for 3-OHBaP. Only 10 min was needed for the analysis. The recovery was 60% for 3-OHBaP and 91 % for 1-OHP, respectively. And the method detection limits were 0.49 mu g/L for 1-OHP and 1.03 mu g/L for 3-OHBaP. The inter- and intra-day relative standard deviations were in the range of 2.8-8.9% for 1-OHP and 9.7-20.8% for 3-OHBaP, respectively. The developed method was successfully used to measure urinary PAH metabolites of student volunteers in a high school. (c) 2006 Elsevier B.V. All rights reserved.
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