Journal
JOURNAL OF CELL BIOLOGY
Volume 173, Issue 4, Pages 545-557Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200601067
Keywords
-
Categories
Funding
- NIGMS NIH HHS [R01 GM069808] Funding Source: Medline
Ask authors/readers for more resources
Mitochondria are distributed within cells to match local energy demands. We report that the microtubule-dependent transport of mitochondria depends on the ability of milton to act as an adaptor protein that can recruit the heavy chain of conventional kinesin-1 (kinesin heavy chain [KHC]) to mitochondria. Biochemical and genetic evidence demonstrate that kinesin recruitment and mitochondrial transport are independent of kinesin light chain (KLC); KLC antagonizes milton's association with KHC and is absent from milton-KHC complexes, and mitochondria are present in k/c(-/-) photo receptor axons. The recruitment of KHC to mitochondria is, in part, determined by the NH2 terminus-splicing variant of milton. A direct interaction occurs between milton and miro, which is a mitochondrial Rho-like GTPase, and this interaction can influence the recruitment of milton to mitochondria. Thus, milton and miro are likely to form an essential protein complex that links KHC to mitochondria for light chain-independent, anterograde transport of mitochondria.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available