Journal
OPTICS EXPRESS
Volume 14, Issue 11, Pages 4727-4735Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.14.004727
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Second harmonic generation microscopy(SHGM) has become widely used to image biological samples. Due to the complexity of biological samples, more and more effort has been put on polarization imaging in SHGM technology to uncover their structures. In this work, we put forward a novel stitching method based on careful mathematical calculation, and accomplish it by rotating laser polarization. We first show its validity in imaging a perfectly synthesized bio-origin polymer poly (3-hyroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx). Then, we test its power by getting a true image of fibrillar collagen structure of rat-tail tendon. (c) 2006 Optical Society of America.
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