Journal
DEVELOPMENT
Volume 133, Issue 11, Pages 2177-2187Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.02386
Keywords
hematopoiesis; endothelial; pronephros; transgenic; zebrafish; Imo2
Categories
Funding
- NHLBI NIH HHS [HL056182] Funding Source: Medline
Ask authors/readers for more resources
The bone morphogenetic protein ( BMP) signaling pathway is essential during gastrulation for the generation of ventral mesoderm, which makes it a challenge to de. ne functions for this pathway at later stages of development. We have established an approach to disrupt BMP signaling specifically in lateral mesoderm during somitogenesis, by targeting a dominant-negative BMP receptor to Lmo2+ cells in developing zebrafish embryos. This results in expansion of hematopoietic and endothelial cells, while restricting the expression domain of the pronephric marker pax2.1. Expression of a constitutively active receptor and transplantation experiments were used to confirm that BMP signaling in lateral mesoderm restricts subsequent hemato-vascular development. The results show that the BMP signaling pathway continues to function after cells are committed to a lateral mesoderm fate, and influences subsequent lineage decisions by restricting hemato-vascular fate in favor of pronephric development.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available