Journal
ONCOGENE
Volume 25, Issue 28, Pages 3894-3904Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.onc.1209426
Keywords
ataxia telangiectasia mutated and rad3-related (ATR); cell cycle checkpoints; DNA damage and repair
Funding
- Cancer Research UK [A5207] Funding Source: Medline
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ATM and ATR are two related kinases essential for signalling DNA damage. Although ATM is thought to be the principle kinase responsible for signalling ionising radiation (IR)-induced DNA damage, ATR also contributes to signalling this form of genotoxic stress. However, the molecular basis of differential ATM and ATR activation in response to IR remains unclear. Here, we report that ATR is recruited to sites of IR-induced DNA damage significantly later than activation of ATM. We show that ATR is recruited to IR-induced nuclear foci in G(1) and S phase of the cell cycle, supporting a role for ATR in detecting DNA damage outside of S phase. In addition, we report that recruitment of ATR to sites of IR-induced DNA damage is concomitant with appearance of large tracts of single-stranded DNA (ssDNA) and that this event is dependent on ATM and components of the Mre11/Rad50/Nbs1 (MRN) protein complex.
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