Journal
BIOPHYSICAL JOURNAL
Volume 90, Issue 11, Pages L80-L82Publisher
CELL PRESS
DOI: 10.1529/biophysj.106.084673
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Funding
- Medical Research Council [G0100471] Funding Source: researchfish
- MRC [G0100471] Funding Source: UKRI
- Medical Research Council [G0100471] Funding Source: Medline
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We apply fluorescence lifetime imaging to the membrane phase-sensing dye di-4-ANEPPDHQ in model membranes and live cells. We show that the 1700 ps lifetime shift between liquid-disordered and liquid-ordered phases offers greater contrast than the 60 nm spectral shift previously reported. Detection of cholesterol-rich membrane microdomains is confirmed by observation of the temperature dependence of membrane order and by cholesterol depletion using methyl-beta-cyclodextrin.
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