Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 385, Issue 4, Pages 753-759Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-006-0419-0
Keywords
microwave-assisted extraction; oleuropein; biophenols; polyphenols; OBPs; olive leaves
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Microwave assistance is proposed for the first time in order to accelerate the extraction of biophenols from olive leaves. Under optimal working conditions, obtained using a multivariate methodology, complete extraction of the target analytes was achieved in 8 min. The extracts required no clean-up nor concentration prior to injection into a chromatograph-photodiode array detector assembly for individual separation-quantification. The optimal extractant (an 80:20 ethanol-water mixture) was also used in the development of a stirring-based extraction method which required around 24 h for complete extraction of the target compounds. These mixtures can be used as replacements for toxic extractants, with a view to exploiting olive leaves in order to obtain biophenols for human use.
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