Journal
TREE PHYSIOLOGY
Volume 26, Issue 6, Pages 767-774Publisher
OXFORD UNIV PRESS
DOI: 10.1093/treephys/26.6.767
Keywords
stable carbon isotope ratio; climate change; diagenesis; translocation
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The stable carbon (C) isotope composition (delta C-13) of tree rings is a powerful metric for reconstructing past physiological responses to climate variation. However, accurate measurement and interpretation are complicated by diagenesis and the translocation of compounds with distinct isotopic signatures. Isolation and analysis of cellulose minimizes these complications by eliminating variation due to biosynthetic pathways; however, isolation of cellulose is time-consuming and has no clear endpoint. A faster and better-defined analytical method is desirable. Our objectives were to determine if there is a direct relationship between the isotopic compositions of whole wood (WW), whole wood treated with solvents to remove mobile extractives (extractive-free wood; EF) and holocellulose (HQ isolated by extractive removal and subsequent bleaching. We also determined if total C concentration could explain the isotopic composition and variation among these three wood components of each sample. A set of wood samples of diverse phylogeny, anatomy and chemical composition, was examined. The mean offset or difference between HC and EF delta C-13 was 1.07 +/- 0.09 parts per thousand and the offset between HC and WW was 1.32 +/- 0.10%, Equivalence tests (with (alpha = 0.05) indicated that the relationship between EF 813 C and HC 813 C had a slope significantly similar to 1 +/- 5.5%, whereas for the WW delta C-13: HC 813 C relationship, the slope was significantly similar to I 10.08%. A regression model using EF delta C-13 to predict HC delta C-13 had a slope of 0.97, which was not significantly different from unity (P = 0.264), whereas the regression for WW had a slope of 0.92 which was significantly different from unity (P = 0.0098). Carbon concentration was correlated with HC:WW offset and cellulose:EF offset (P = 0.0501 and 0.007, respectively), but neither relationship explained much of the variation (r(2) = 0.12 and 0.14, respectively). We suggest that HC extraction is unnecessary for most analyses of tree-ring delta C-13; a simple solvent extraction is a suitable alternative for many applications.
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