Journal
JOURNAL OF VIROLOGY
Volume 80, Issue 12, Pages 5919-5926Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.00046-06
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Funding
- Intramural NIH HHS Funding Source: Medline
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Hepatitis E virus replicons containing the neomycin resistance gene expressed from open reading frames (ORFs) 2 and 3 were transfected into Huh-7 cells, and stable cell lines containing functional replicons were selected by constant exposure to G418 sulfate. Northern blot analyses detected full-length replicon RNA and a single subgenomic RNA. This subgenomic RNA, which was capped, initiated at nucleotide 5122 downstream of the first two methionine codons in ORF3 and was bicistronic; two closely spaced methionine codons in different reading frames were used for the initiation of ORF3 and ORF2 translation.
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