Journal
FEMS MICROBIOLOGY LETTERS
Volume 259, Issue 1, Pages 53-59Publisher
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2006.00246.x
Keywords
S-adenosylmethionine; streptomycin; adpA transcription; Streptomyces griseus
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S-adenosylmethionine (SAM), the major methyl donor in diverse biological processes, was previously documented as a regulator for secondary metabolism in Streptomyces. The present study demonstrates that exogenous SAM, in a quantity as low as 10 mu M, enhanced streptomycin production and adpA transcription in both Streptomyces griseus wild-type strain and mutant HO1, which displays no ArpA repression on the adpA promoter. SAM also enhanced xylE expression driven by the promoter of adpA or strR in a heterologous host, S. lividans. This report substantiates that exogenous SAM promotes adpA transcription in S. griseus, which is involved in the SAM-mediated promotion of streptomycin, and that the mechanism underlying this event is shared in S. lividans.
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