Journal
GENE THERAPY
Volume 13, Issue 12, Pages 991-994Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.gt.3302753
Keywords
monocyte-derived human DCs; lentiviral vectors; virion-like particles; HIV-1; SIVMAC
Ask authors/readers for more resources
Modification of dendritic cells (DCs) is a promising avenue for gene therapy purposes, given the versatility and the multiplicity of functions of these cells. In this study, we show that preincubation of monocyte-derived DCs with low amounts of non-infectious virion-like particles derived from the simian immunodeficiency virus (SIVMAC VLPs) increases up to 10-fold the efficiency of transduction by HIV-1 lentiviral vectors at low multiplicity of infections yielding up to 90% of transduced cells, in the absence of alterations of DCs behavior. This effect is restricted to DCs and specified by the viral accessory protein Vpx. Thus, preincubation with empty VLPs of SIVMAC can be used in transduction protocols to increase the efficacy of HIV-1-mediated modification of DCs.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available