Journal
MOLECULAR ECOLOGY NOTES
Volume 6, Issue 2, Pages 440-442Publisher
WILEY
DOI: 10.1111/j.1471-8286.2006.01264.x
Keywords
aphids; Aphis fabae complex; cross-species amplification; host plant; microsatellites; multiplex PCR
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Aphis fabae includes four morphological cryptic subspecies, which are mostly identified by their partially distinct secondary host range. To determine the extent of gene flow and isolation between these four taxa, we isolated and characterized 12 microsatellite loci from Aphis fabae fabae and tested cross-species amplification of eight loci from the closely related species Aphis gossypii. Using eight previously described microsatellite loci, we have developed the polymerase chain reaction (PCR) multiplexing of 24 loci, which were separated in tree sets and five PCRs. These sets of microsatellite loci provide high throughput capacity for large-scale population genetic studies at a minimum cost.
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