Journal
JOURNAL OF IMMUNOLOGY
Volume 176, Issue 11, Pages 6879-6887Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.176.11.6879
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Funding
- NIAID NIH HHS [T32 AI055428] Funding Source: Medline
- NIAMS NIH HHS [T32-AR007442] Funding Source: Medline
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Tolerance to dsDNA is broken in mice with a high-affinity anti-DNA H chain transgene, 56R, on the C57BL/6 background (B6.56R)). B6.56R produce more anti-dsDNA Abs than BALBc.56R. To investigate how anti-DNA Abs are regulated on the B6 background, phenotypic and genetic studies were performed. B6.56R have reduced numbers of B cells and phenotypically altered B cell subsets, including relative increases in the proportions of IgM-negative bone marrow B cells, cells with a marginal zone phenotype, and cells with a transitional T3 phenotype. The peripheral B cell repertoire in B6.56R is restricted: most B cells express the 56R H chain and use a similar, limited subset of editor L chains. DNA binding is more common in B6.56R because the repertoire is shifted toward L chains that are more permissive for DNA binding. H chain editing is also observed and is increased in spontaneous as compared with LPS hybridomas. A subset of spontaneous hybridomas appears to lack H chain expression.
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