Journal
HUMAN REPRODUCTION
Volume 21, Issue 6, Pages 1583-1590Publisher
OXFORD UNIV PRESS
DOI: 10.1093/humrep/del027
Keywords
ejaculated human spermatozoa; male fertility; mRNA profile; reproduction; serial analysis of gene expression
Categories
Ask authors/readers for more resources
BACKGROUND: Accumulated evidence proves that mature spermatozoa contain a complex yet specific array of mRNA, which could provide information on the past events of spermatogenesis. OBJECTIVE: To quantitatively microdissect these mRNA transcripts by a digital approach. METHODS: Serial analysis of gene expression (SAGE) was used to study the mRNA transcripts from ejaculate of a fertile individual and of a pool of 10 fertile men. Online DAVID software suite was also utilized to cluster the UniGene data. RESULTS: A SAGE library from the individual produced 20 237 raw tags representing 2459 unique tags and that from pooled 10 men generated 21 052 raw tags representing 2712 unique tags. When the unique tags occurring A times were analysed, 564 overlapping tags were produced by 638 unique tags from the individual and 682 from the pooled library. Fifty-four of these overlapped tags were considered to be novel genes. Online analysis of the overlapping tags revealed 25 functional gene groups, with the dominant one comprising 96 nuclear protein genes involving transcription and transcription regulation and also a group with 84 ribosomal subunit genes involving protein synthesis. CONCLUSION: A SAGE analysis of ejaculate from fertile men has revealed a large number of transcripts, which occur in steady frequencies and probably have important roles in spermatogenesis and fertilization.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available