Journal
PLOS GENETICS
Volume 2, Issue 6, Pages 811-825Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pgen.0020091
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Funding
- Medical Research Council [MC_U117560482] Funding Source: researchfish
- MRC [MC_U117560482] Funding Source: UKRI
- Medical Research Council [MC_U117560482] Funding Source: Medline
- NICHD NIH HHS [1 R01 HD4 2276-01, R01 HD042276] Funding Source: Medline
- Wellcome Trust Funding Source: Medline
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We present here the results of forward and reverse genetic screens for chemically-induced mutations in Xenopus tropicalis. In our forward genetic screen, we have uncovered 77 candidate phenotypes in diverse organogenesis and differentiation processes. Using a gynogenetic screen design, which minimizes time and husbandry space expenditures, we find that if a phenotype is detected in the gynogenetic F2 of a given F1 female twice, it is highly likely to be a heritable abnormality (29/29 cases). We have also demonstrated the feasibility of reverse genetic approaches for obtaining carriers of mutations in specific genes, and have directly determined an induced mutation rate by sequencing specific exons from a mutagenized population. The Xenopus system, with its well-understood embryology, fate map, and gain-of-function approaches, can now be coupled with efficient loss-of-function genetic strategies for vertebrate functional genomics and developmental genetics.
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