Journal
MOLECULAR BIOLOGY REPORTS
Volume 33, Issue 2, Pages 83-89Publisher
SPRINGER
DOI: 10.1007/s11033-005-2311-y
Keywords
bacterioMatch two-hybrid system; cytoskeleton; partner proteins; TIMAP
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Funding
- NHLBI NIH HHS [HL58064, HL67307, HL68062, HL80675] Funding Source: Medline
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BacterioMatch Two-Hybrid System (Stratagene) was applied in order to identify potential human TIMAP interaction proteins in the lung. TIMAP highly expressed in endothelial cells and may be involved in endothelial cytoskeletal and barrier regulation. Seven TIMAP interacting partner proteins were identified. Four of identified proteins: cystein and glycine-rich protein 1, eukaryotic translation elongation factor 2, U5 snRNP-specific protein 116 kD, and solute carrier family 3 member 2 are involved in actin cytoskeleton organization, cell adhesion or translation and transcriptional regulation.
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