Journal
GENOMICS
Volume 87, Issue 6, Pages 693-703Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygeno.2006.02.003
Keywords
blood; humans; gene expression; microarrays; genome; T cells; B cells; NK cells; neutrophils; migraine; stroke; Tourette
Funding
- NIEHS NIH HHS [P01 ES 11269] Funding Source: Medline
- NINDS NIH HHS [NS 028167, NS042774, NS043252, NS044283] Funding Source: Medline
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Blood genomic profiling has been applied to disorders of the blood and various organ systems including brain to elucidate disease mechanisms and identify surrogate disease markers. Since most studies have not examined specific cell types, we performed a preliminary genomic survey of major blood cell types from normal individuals using microarrays. CD4(+) T cells, CD8(+) T cells, CD19(+) B cells, CD56(+) natural killer cells, and CD14(+) monocytes were negatively selected using the RosetteSep antibody cocktail, while polymorphonuclear leukocytes were separated with density gradient media. Genes differentially expressed by each cell type were identified. To demonstrate the potential use of such cell subtype-specific genomic expression data, a number of the major genes previously reported to be regulated in ischemic stroke, migraine, and Tourette syndrome are shown to be associated with distinct cell populations in blood. These specific gene expression, cell-type-related profiles will need to be confirmed in larger data sets and could be used to study these and many other neurological diseases. (c) 2006 Elsevier Inc. All rights reserved.
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