Journal
FEMS MICROBIOLOGY LETTERS
Volume 259, Issue 1, Pages 74-80Publisher
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2006.00247.x
Keywords
Bacillus cereus; cytotoxin K; cytK-1 gene; cytK-2 gene; diarrhoeal food poisoning; PCR; detection
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Bacillus cereus is the causative agent of gastrointestinal diarrhoea. At least three known enterotoxins may be involved in this syndrome: nonhaemolytic (Nhe) enterotoxin, Hbl enterotoxin and cytotoxin K. Two different forms were recently described for cytotoxin K, encoded by cytK-1 and cytK-2 genes. The CytK-1 toxin appeared to carry a high toxicity, but there is currently no method available to rapidly detect and discriminate the B. cereus strains able to produce this CytK-1 form. In this study, a duplex PCR assay was developed and validated on 162 known cytotoxin-containing strains. This PCR method is the first molecular tool to provide rapid detection and discrimination of cytK-1- and cytK-2-carrying B. cereus strains.
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