Journal
BIOPHYSICAL JOURNAL
Volume 90, Issue 12, Pages 4428-4436Publisher
CELL PRESS
DOI: 10.1529/biophysj.105.080283
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Funding
- Intramural NIH HHS Funding Source: Medline
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Vesicles containing ternary mixtures of diphytanoylphosphatidylcholine, dipalmitoylphosphatidylcholine (DPPC), and cholesterol produce coexisting liquid phases over an unusually large range of temperature and composition. Liquid domains persist well above the DPPC chain melting temperature (41 degrees C), resulting in a closed-loop miscibility gap bounded by two critical points at fixed temperature. Quantitative tie-lines are determined directly from H-2 NMR spectra using a novel analysis, and are found to connect a liquid-disordered phase rich in diphytanoyl PC with a liquid-ordered phase rich in DPPC. The direction of the tie-lines implies that binary DPPC/cholesterol mixtures are in one uniform phase above 41 degrees C. All H-2 NMR results for tie-lines are verified by independent fluorescence microscopy results.
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