Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 837, Issue 1-2, Pages 125-132Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2006.04.027
Keywords
DCA; MA; SA; GC-ECNCI-MS; PFB-Br; tyrosine metabolites
Funding
- NIEHS NIH HHS [R01 ES007355, P42 ES07375] Funding Source: Medline
- PHS HHS [M01 000082] Funding Source: Medline
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We developed a sensitive method to quantitate the tyrosine metabolites maleylacetone (MA) and succinylacetone (SA) and the tyrosine metabolism inhibitor dichloroacetate (DCA) in biological specimens. Accumulation of these metabolites may be responsible for the toxicity observed when exposed to DCA. Detection limits of previous methods are 200 ng/mL (1.2 pmol/mu L) (MA) and 2.6 mu g/mL (16.5 pmol/mu L) (SA) but the metabolites are likely present in lower levels in biological specimens. To increase sensitivity, analytes were extracted from liver, urine, plasma and cultured nerve cells before and after dosing with DCA, derivatized to their pentafluorobenzyl esters, and analyzed via GC-MS/MS. (c) 2006 Elsevier B.V. All rights reserved.
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