Journal
WORLD JOURNAL OF GASTROENTEROLOGY
Volume 12, Issue 21, Pages 3359-3367Publisher
BAISHIDENG PUBLISHING GROUP INC
DOI: 10.3748/wjg.v12.i21.3359
Keywords
solanine; hepatocarcinomatic cell; Ca2+ in the cell; membrane potential; laser confocal scanning microscopy
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AIM: To observe the effect of solanine on the membrane potential of mitochondria in HepG(2) cells and [Ca2+], in the cells, and to uncover the mechanism by which solanine induces apoptosis. METHODS: HepG(2) cells were double stained with AO/EB, and morphological changes of the cells were observed using laser confocal scanning microscopy (LCSM). HepG(2) cells were stained with TMRE, and change in the membrane potential of mitochondria in the cells were observed using LCSM. HepG(2) cells were double stained with Fluo-3/AM, and change of [Ca2+](i) in the cells were observed using LCSM. HepG(2) cells were double stained with TMRE and Fluo-3/AM, and both the change in membrane potential of mitochondria and that of [Ca2+](i) in the cells were observed using LCSM. RESULTS: Cells in treated groups showed typical signs of apoptosis. Staining with TMRE showed that solanine could lower membrane potential; staining with Fluo-3/AM showed that solanine could increase the concentration of Ca2+ in tumor cells; and those of double staining with TMRE and Fluo-3/AM showed that solanine could increase the concentration of Ca2+ in the cells at the same time as it lowered the membrane potential of mitochondria. CONCLUSION: Solanine opens up the PT channels in the membrane by lowering the membrane potential, leading to Ca2+ being transported down its concentration gradient, which in turn leads to the rise of the concentration of Ca2+ in the cell, turning on the mechanism for apoptosis. (C) 2006 The WJG Press. All rights reserved.
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