Journal
FEBS LETTERS
Volume 580, Issue 14, Pages 3545-3550Publisher
WILEY
DOI: 10.1016/j.febslet.2006.05.033
Keywords
polypurine tract; triple-helix; sequence-specific RNA degradation; reverse transcriptase-RNase H; siRNA
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We describe inhibition of HIV replication by a partially double-stranded 54mer oligodeoxynucleotide, ODN, which consists of an antisense strand targeting the highly conserved polypurine tract, PPT, of HIV, and a second strand, compatible with triple-helix formation. Upon treatment of HIV-infected cells with ODN early after infection no viral nucleic acids, syncytia or p24 viral antigen expression was observed. The ODN-mediated effect was highly sequence-specific. The ODN against HIV-IIIB was effective preferentially against its homologous PPT and less against the PPT of HIV-BaL differing in two of 24 nucleotides and vice versa. It may be interesting mechanistically as an antiviral drug. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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