Journal
JOURNAL OF CELL SCIENCE
Volume 119, Issue 12, Pages 2486-2496Publisher
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.02967
Keywords
Arabidopsis; meiosis; recombination; synapsis
Categories
Funding
- Austrian Science Fund FWF [P 18036] Funding Source: Medline
- Biotechnology and Biological Sciences Research Council [BBS/E/J/00000594] Funding Source: Medline
- Biotechnology and Biological Sciences Research Council [BBS/E/J/00000594] Funding Source: researchfish
Ask authors/readers for more resources
Mnd1 has recently been identified in yeast as a key player in meiotic recombination. Here we describe the identification and functional characterisation of the Arabidopsis homologue, AtMND1, which is essential for male and female meiosis and thus for plant fertility. Although axial elements are formed normally, sister chromatid cohesion is established and recombination initiation appears to be unaffected in mutant plants, chromosomes do not synapse. During meiotic progression, a mass of entangled chromosomes, interconnected by chromatin bridges, and severe chromosome fragmentation are observed. These defects depend on the presence of SPO11-1, a protein that initiates recombination by catalysing DNA double-strand break (DSB) formation. Furthermore, we demonstrate that the AtMND1 protein interacts with AHP2, the Arabidopsis protein closely related to budding yeast Hop2. These data demonstrate that AtMND1 plays a key role in homologous synapsis and in DSB repair during meiotic recombination.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available