Journal
JOURNAL OF COLLOID AND INTERFACE SCIENCE
Volume 298, Issue 2, Pages 825-831Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jcis.2006.03.045
Keywords
glass surface; aminosilane; microarrays; XPS; molecular orientation; Raman
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The surface properties and structure of mono-, di-, and tri-aminosilane treated glass surfaces were investigated using surface analytical techniques including X-ray photoelectron spectroscopy (XPS), Raman spectroscopy, atomic force microscopy (AFM), and streaming potential. An optimized dip-coating process was demonstrated to produce roughly silane monolayer coverage on the glass surface. The surface charge measurements indicated that ammosilanization converts the class surface from negative to positive potentials at neutral pH values. Higher positive streaming potential was observed for tri- compared with mono- and di-aminosilane treated glass surfaces. For all aminosilane treated glass samples. the high-resolution N 1s XPS spectra indicated a preferential orientation of the protonated amino-groups towards the glass surface whereas the free amino groups were protruding outward. This study aimed to obtain uniform, reproducibly thin, strongly adhering, internally cross-linked, and high positively charged aminosilane-coated glass surfaces for the attachment of DNA fragments used in microarraying experiments. (c) 2006 Elsevier Inc. All rights reserved.
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