Journal
TALANTA
Volume 69, Issue 4, Pages 841-847Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2005.11.028
Keywords
on-line derivatization; sequential injection; LC; amino acids; pharmaceuticals
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The principle of sequential injection (SI) was exploited to develop a fully automated pre-column derivatization procedure combined online to liquid chromatography (LC). Using SI-LC derivatization 14 amino acids were determined fluori metrically in pharmaceuticals with o-phthaldialdehyde (OPA) as the derivatization reagent. The SI system was used for the handling of samples and reagents, on-line mixing and introduction to the LC injection system. Chemical (pH and reagents concentrations) and instrumental variables (sample and reagent volumes, reaction time and flow rate) were optimized to attain the highest reaction yield and detector signal. Reversed phase chromatographic resolution of 14 amino acids was achieved within 35 min using gradient elution. The automated operation of the coupled SI-LC system resulted in very satisfactory performance. The method was applied for the simultaneous determination of amino acids in pharmaceutical formulations. (c) 2005 Elsevier B.V. All rights reserved.
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