Journal
ANALYTICA CHIMICA ACTA
Volume 570, Issue 2, Pages 165-169Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2006.04.010
Keywords
bienzyme biosensor; aspartame; ethanol; graphite epoxy composite electrode (GECE)
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A gelatin membrane with carboxyl esterase and alcohol oxidase was subsequently integrated onto the surface of a graphite epoxy composite electrode (GECE). The developed biosensors showed linearity in the range of 2.5-400 mu M for aspartame and 2.5-25 mu M for ethanol with response times of 170 and 70 s for each analyte, respectively. The resulting bienzyme biosensor was used for aspartame detection in diet coke samples and ethanol detection in beer and wine samples. From the obtained results, it can be concluded that the developed biosensor is a selective, practical and economic tool for aspartame and ethanol detection in real samples. (c) 2006 Elsevier B.V. All rights reserved.
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