Journal
VACCINE
Volume 24, Issue 25, Pages 5277-5293Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2006.03.013
Keywords
Neisseria meningitidis; Neisseria lactamica; vaccine; outer membrane vesicle; proteomics; tandem mass spectrometry
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Vaccines to prevent meningococcal disease have been developed from the outer membrane vesicles (OMVs) of Neisseria meningitidis and the related commensal organism Neisseria lactarnica. In addition to lipopolysaccharide and the major porins, these vaccines contain a large number of proteins that are incompletely characterised. Here we describe comparative proteomic analyses of the N. lactamica OMV vaccine and OMVs from a serogroup B strain of N. meningitidis. Tandem mass-spectrometry data for trypsinised N. lactamica OMV vaccine were matched to an incompletely assembled genome sequence from the same strain to give 65 robust protein identifications and a further 122 single- or two-peptide matches. Fifty-seven N. meningitidis K454 proteins were identified robustly (and a further 68 from single- or two-peptide matches) by inference from the N. meningitidis MC58 genome. The results suggest that OMVs have a hitherto unappreciated complexity and pinpoint novel candidate antigens for further characterisation. (c) 2006 Elsevier Ltd. All rights reserved.
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