Journal
EMBO JOURNAL
Volume 25, Issue 12, Pages 2768-2780Publisher
WILEY
DOI: 10.1038/sj.emboj.7601164
Keywords
rNA silencing; small RNA binding; viral suppressors
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Funding
- NIAID NIH HHS [AI43288, R21 AI043288, R01 AI043288, R37 AI043288] Funding Source: Medline
- NIGMS NIH HHS [R01 GM053190, GM053190] Funding Source: Medline
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RNA silencing is an evolutionarily conserved system that functions as an antiviral mechanism in higher plants and insects. To counteract RNA silencing, viruses express silencing suppressors that interfere with both siRNA- and microRNA-guided silencing pathways. We used comparative in vitro and in vivo approaches to analyse the molecular mechanism of suppression by three well-studied silencing suppressors. We found that silencing suppressors p19, p21 and HC-Pro each inhibit the intermediate step of RNA silencing via binding to siRNAs, although the molecular features required for duplex siRNA binding differ among the three proteins. None of the suppressors affected the activity of preassembled RISC complexes. In contrast, each suppressor uniformly inhibited the siRNA- initiated RISC assembly pathway by preventing RNA silencing initiator complex formation.
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