Journal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 102, Issue 1, Pages 41-45Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1263/jbb.102.41
Keywords
bioartificial liver; hepatocytes; bioreactor; galactose; vegetable sponge
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A galactose moiety was introduced into the fiber surface of a vegetable sponge by the covalent binding of lactobionic acid. The galactosylated sponge was used as scaffold for the culture of rat hepatocytes in a packed-bed bioreactor. Hepatocytes could be dynamically seeded into and uniformly distributed throughout the scaffold, and the immobilized cells maintained high albumin and urea production rates during long-term perfusion culture. The hepatocytes showed an increasing albumin production rate from 49 to log mu g/10(6) cells/d over the 7-d culture.
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