Journal
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
Volume 69, Issue 1-3, Pages 62-72Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.pmpp.2007.01.001
Keywords
oak tree; Phytophthora cinnamomi; cinnamyl alcohol dehydrogenase; defence response
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Funding
- Fundação para a Ciência e a Tecnologia [POCTI/AGR/34389/2000, SFRH/BD/82666/2011] Funding Source: FCT
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A gene encoding a potential NADPH-dependent cinnamyl alcohol dehydrogenase (QsCAD1) (GenBank accession no: AY362455) was identified in Quercus suber (cork oak). Its complete cDNA sequence was obtained by RACE-PCR, starting from total RNA extracted from roots of seedlings of Q. suber, infected with Phytophthora cinnamomi, the causal agent of the decline and sudden death of Q. suber and Quercus ilex subsp. rotundifolia in the Iberian Peninsula. Sequence information to perform the RACE-PCR was acquired from a polymorphic fragment (C9), specifically identified by cDNA-AFLP, in leaves of epicormic shoots of a cork oak tree that suffered sudden death. RT-PCR and hybridization analysis showed that the QsCAD1 gene is up-regulated in root seedlings of Q. suber infected with P. cinnamomi. QsCAD1 has a high structural homology with VR-ERE (Vigna radiata), an enzyme that detoxifies eutypine (produced by Eutypa lata, the causal agent of eutypa dieback of grapevines), to eutypinol, and with QrCAD1 (Q. ilex subsp. rotundifolia), EgCAD1 (Eucalyptus gunnii), MdCAD1 (Malus x domestica). Taken together, these results suggest that these enzymes, and namely QsCAD 1 belong to a new group of CAD potentially involved in deactivation of toxins produced by phytopathogens. (c) 2007 Elsevier Ltd. All rights reserved.
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