Journal
JOURNAL OF BIOMEDICAL SCIENCE
Volume 13, Issue 4, Pages 593-595Publisher
SPRINGER
DOI: 10.1007/s11373-006-9078-6
Keywords
acid-base regulation; bicarbonate transporter; sodium bicarbonate symporter
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Funding
- NIDDK NIH HHS [DK 061418, R01 DK061418] Funding Source: Medline
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In this study, we examined the tissue-specific expression of two electroneutral Na/HCO3 cotransporter (NBCn1) variants that differ from each other by the presence of the N-terminal 123 amino acids (cassette II). A rat Northern blot with the probe to nucleotides encoding cassette II detected a 9 kb NBCn1 mRNA strongly in the heart and weakly in skeletal muscles, but absent from most of the tissues including kidney, brain, and pancreas. In the rat heart, PCR with primers flanking cassette II preferentially amplified a DNA fragment that lacked cassette II. However, in the human heart, PCR preferentially amplified a fragment that contained cassette II. This larger PCR product was found virtually in all regions of the human cardiovascular system with strong amplification in the apex, atrium, and atrioventricular nodes. These findings indicate that the variant containing cassette II is almost absent in tissues including brain, kidney, and pancreas, where NBCn1 has been extensively examined.
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