Journal
JOURNAL OF SEPARATION SCIENCE
Volume 29, Issue 10, Pages 1508-1516Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200600129
Keywords
dynamic HPLC; chiral stationary phases; stereomutation, enantiomerization; computer simulation
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Dynamic HPLC on enantioselective stationary phases has become a well-established technique to investigate chiral molecules with internal motions that result in stereoinversion and occur on the time scale of the separation process. Kinetic parameters for the on-column interconversion phenomena can be extracted from experimental peak profiles by computer simulation or by direct calculation methods. The technique has been used in a wide range of temperatures and is complementary in scope to dynamic NMR spectroscopy.
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