Journal
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
Volume 81, Issue 7, Pages 1190-1197Publisher
WILEY
DOI: 10.1002/jctb.1512
Keywords
lactic acid; kinetic model; Lactobacillus casei; whey
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The biomass growth, lactic acid production and lactose utilisation kinetics of lactic acid production from whey by Lactobacillus casei was studied. Batch fermentation experiments were performed at controlled pH and temperature with six different initial whey lactose concentrations (9-77 g dm(-3)) in a 3 dm(3) working volume bioreactor. Biomass growth was well described by the logistic equation with a product inhibition term. In addition, biomass and product inhibition effects were defined with corresponding power terms, which enabled adjustment of the model for low- and high-substrate conditions. The Luedeking-Piret equation defined the product formation kinetics. Substrate consumption was explained by production rate and maintenance requirements. A maximum productivity of 2.5 gdm(-3)h(-1) was attained with an initial lactose concentration of 35.5 g dm(-3). (c) 2006 Society of Chemical Industry.
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