Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 66, Issue 1, Pages 1-20Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2006.02.016
Keywords
wastewater; bacterial communities; pathogen detection; molecular techniques; nucleic acid finger printing; PCR; microarray FISH; lab-on-chip
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Traditionally, the detection of pathogens in water, wastewater, and other environmental samples is restricted by the ability to culture such organisms from complex environmental samples. During the last decade the use of molecular methods have supplied the means for examining microbial diversity and detecting specific organisms without the need for Cultivation. The application of molecular techniques to the study of natural and engineered environmental systems has increased our insight into the vast diversity and interaction of microorganisms present in complex environments. In this paper, we will review the current and emerging molecular approaches for characterizing microbial community composition and structure in wastewater processes. Recent studies show that advances in microarray assays are increasing our capability of detecting hundreds and even thousands of DNA sequences simultaneously and rapidly. With the current progress in microfluidics and optoelectronics, the ability to automate a detection/identification system is now being realized. The status of such a system for wastewater monitoring is discussed. (c) 2006 Elsevier B.V. All rights reserved.
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