Journal
BIOLOGICAL & PHARMACEUTICAL BULLETIN
Volume 29, Issue 7, Pages 1516-1520Publisher
PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/bpb.29.1516
Keywords
nuclear factor kappa B (NF kappa B) decoy; folate receptor; folate-linked nanoparticle; macrophage; rheumatoid arthritis
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Activated macrophages are the key effector cells in rheumatoid arthritis (RA) and secrete multiple mediators of inflammation including proinflammatory cytokines. We investigated delivery of a nuclear factor kappa B (NF kappa B) decoy by folate-linked lipid-based nanoparticles (NP-F) into murine macrophages. The expression of folate receptor (FR) in RAW264.7 cells activated by lipopolysaccaride was confirmed by strong expression of FR mRNA, and association of FITC-labeled folate-BSA conjugate. When transfected via NP-F, the NF kappa B decoy was strongly detected in the cytoplasm, and an inhibitory effect on the translocation of NF kappa B into the nucleus was observed at 0.03 mu m of the decoy, suggesting that NP-F effectively delivered the NF kappa B decoy into the cytoplasm. This information is of value for the design of NF kappa B decoy carrier systems targeting FR in activated macrophages in gene therapy for autoimmune diseases such as RA.
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