Antioxidant and free radical scavenging activity of an aqueous extract of Coleus aromaticus

The leaves of Coleus aromaticus Benth., (Larniaceae), commonly called Indian Borage, are often eaten raw with bread and butter. The chopped leaves are also used as a substitute for sage (Salvia officinalis Linn.) in stuffing. In the present study, the antioxidant potency of freeze-dried aqueous extract of C aromaticus was investigated, employing various established in vitro systems, such as the beta-carotene-linoleate model system, 1,1-diphenyl-2-picrylhydrazyl (DPPH)/superoxide/nitric oxide radical scavenging, reducing power, and iron ion chelating activity. Freeze-dried aqueous extract of C aromaticus (CAE) showed notable inhibitory activity in the beta-carotene-linoleate model system. Furthermore, CAE exhibited a moderate concentration-dependent inhibition of the DPPH radical. The multiple antioxidant activity of CAE was evident as it showed significant reducing power, superoxide scavenging ability, nitric oxide-scavenging activity and also ferrous ion chelating potency. The data obtained in the in vitro models clearly establish the antioxidant potency of freeze-dried extract of C aromaticus. (c) 2005 Elsevier Ltd. All rights reserved.