Journal
ANALYTICAL CHEMISTRY
Volume 78, Issue 13, Pages 4727-4731Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac0521955
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Two label-free electrochemical methods for the detection of human alpha-thrombin using a water-soluble, ferrocene-functionalized polythiophene transducer and a single-stranded oligonucleotide aptamer probe are described. The first approach is a direct method in which the recorded current decreases upon addition of the targeted protein. The second one requires more steps and the additional utilization of PNA probes and nuclease enzyme. This indirect method leads to an increase of the electrical signal as a function of the concentration of human alpha-thrombin with a detection limit of 75 fmol.
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