Journal
DEVELOPMENTAL CELL
Volume 11, Issue 1, Pages 21-32Publisher
CELL PRESS
DOI: 10.1016/j.devcel.2006.05.012
Keywords
-
Categories
Ask authors/readers for more resources
CLASPs are mammalian microtubule-stabilizing proteins that can mediate the interaction between distal microtubule ends and the cell cortex. Using mass spectrometry-based assays, we have identified two CLASP partners, LL5 beta and ELKS. LL5 beta and ELKS form a complex that colocalizes with CLASPS at the cortex of HeLa cells as well as at the leading edge of motile fibroblasts. LL5 beta is required for cortical CLASP accumulation and microtubule stabilization in HeLa cells, while ELKS plays an accessory role in these processes. LL5 beta is a phosphatidylinositol-3,4,5-triphosphate (PIP3) binding protein, and its recruitment to the cell cortex is influenced by PI3 kinase activity but does not require intact microtubules. Cortical clusters of LL5 beta and ELKS do not overlap with focal adhesions but often form in their vicinity and can affect their size. We propose that LL5 beta and ELKS can form a PIP3-regulated cortical platform to which CLASPs attach distal microtubule ends.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available